Proteomics

Orbitrap Fusion Lumos Tribrid Mass Spectrometer

The Thermo Orbitrap Fusion Lumos Tribrid MS allows systems biology researchers to test new limits of quantitation and protein characterization. It incorporates the brightest ion source, a segmented quadrupole mass filter with improved selectivity and ion transmission, Advanced Vacuum Technology for improved ion transmission to the Orbitrap mass analyzer and higher-capacity ETD fragmentation. Combined, these hardware improvements enable the new tribrid instrument to excel in the most challenging applications, including analysis of low level PTMs (phosphorylation, glycosylation), multiplexed relative quantitation using isobaric tags (TMT, iTRAQ) and more accurate Synchronous Precursor Selection-MS3 method, protein-protein interaction using MS-cleavable crosslinking reagents, as well as Data-Independent Acquisition (DIA).

Q Exactive HF

We acquired Thermo Q Exactive plus in 2014 and upgraded to Q Exactive HF in 2015. The Q Exactive HF is a hybrid quadrupole-Orbitrap mass spectrometer which has the ability to routinely and confidently identify, quantify, and confirm in a single analysis. The high-field (HF) orbitrap with 240,000 maximum resolution ensures maximum ID confidence and faster scan speed. We are able to run data-dependent analysis (DDA), targeted SIM, targeted MS2, data-independent analysis (DIA) and parallel reaction monitoring (PRM) within the same mass spectrometer, which provide reproducible quantitation results while delivering complete qualitative confidence.

Agilent 1290 Infinity II LC and 6546 Q-TOF MS

Our newest addition is the Agilent 6546 Quadrupole Time of Flight (Q-TOF) MS. With simultaneous high sensitivity, high dynamic range (5 orders), high resolution (30K for low mass / 60K for high mass), isotope fidelity (within 5%), and accurate mass (sub-ppm) measurements, the resulting high data quality provides the level of confidence for comprehensive workflows in metabolomics research, such as metabolites profiling and qualitative flux analysis.