RESEARCH GROUP

Dr. Giordano Pula

Education
1992	Scientific Diploma Scientific School "Belfiore", Mantova, Italy
1998	Degree in Molecular Biology University of Parma, Italy
2000-2003	PhD in Molecular Pharmacology, Department of Pharmacology, University of Bristol, UK
Previous Appointment
1998-2000	Molecular Pharmacology Unit GlaxoSmithKline Research Centre, Verona, Italy
2003-2006	Postdoctoral Research Associate Department of Pharmacology, University of Bristol, UK
2006-2007	Postdoctoral Research Associate Randall Division, King’s College, London, UK
2007-2009	Postdoctoral Fellow Cardiovascular Division, King’s College, London, UK

Publications

1	Prokopi M, Pula G, Mayr U, Devue C, Gallagher J, Xiao Q, Boulanger CM, Westwood N, Urbich C, Willeit J, Steiner M, Breuss J, Xu Q, Kiechl S, Mayr M. Proteomic analysis reveals presence of platelet microparticles in endothelial progenitor cell cultures. *Blood. 2009; 114* : 723. [Full Text] [PubMed] [DOI]
2	Pula G, Mayr U, Evans C, Prokopi M, Vara DS, Yin X, Astroulakis Z, Xiao Q, Hill J, Xu Q, Mayr M. Proteomics identifies thymidine phosphorylase as a key regulator of the angiogenic potential of colony-forming units and endothelial progenitor cell cultures. *Circ Res. 2009; 104* : 32-40. [Full Text] [PubMed] [DOI]
3	Pula G, Poole AW. Critical roles for the actin cytoskeleton and cdc42 in regulating platelet integrin alpha2beta1. *Platelets*. 2008; 19 : 199-210. [PubMed] [DOI]
4	Pula G, Krause M. Role of Ena/VASP Proteins in Homeostasis and Disease. *Handb Exp Pharmacol. 2008; 186* : 39-65. [PubMed] [DOI]
5	Pula G, Perera S, Sidibe A, Prokopi M, Mayr M. Proteomic analysis of secretory proteins and vesicles in vascular research. *Proteomics Clin Appl*. 2008; 2 : 882-891. [Full Text] [DOI]
6	Pula G, Schuh K, Nakayama K, Nakayama KI, Walter U, Poole AW. PKCdelta regulates collagen-induced platelet aggregation through inhibition of VASP-mediated filopodia formation. *Blood. 2006; 108* : 4035-4044.
7	Wenworth JKT, Pula G and Poole AW. Vasodilator-stimulated phosphoprotein (VASP) is phosphorylated on Ser157 by protein kinase C-dependent and -independent mechanisms in thrombin-stimulated human platelets. *Biochemical Journal. 2006; 393* : 555-564.
8	Pula G, Crosby D, Baker J, Poole AW. Functional interaction of PKCalpha with the tyrosine kinases Syk and Src in human platelets. *J. Biol. Chem. 2005; 280* : 7194-7205.
9	Pula G, Mundell SJ, Roberts PJ and Kelly E. Analysis of mGluR1a constitutive internalization using a pulse-chase enzyme-linked immuno-sorbant assay (ELISA). *Journal of Biochemical and Biophysical Methods*. 2005; 64 : 167-181.
10	Poole AW, Pula G, Hers I, Crosby D, Jones ML. PKC-interacting proteins: from function to pharmacology. *Trends Pharmacol. Sci*. 2004; 25 : 528-535.
11	Pula G, Mundell SJ, Roberts PJ, Kelly E. Agonist-independent internalization of metabotropic glutamate receptor 1a is arrestin- and clathrin-dependent and is suppressed by receptor inverse agonists. *J. Neurochem*. 2004; 89 : 1009-1020.
12	Mundell SJ, Pula G, McIlhinney RA, Roberts PJ, Kelly E. Desensitization and internalization of metabotropic glutamate receptor 1a following activation of heterologous Gq/11-coupled receptors. *Biochemistry*. 2004; 43 : 7541-7551.
13	Mundell SJ, Pula G, More JC, Jane DE, Roberts PJ, Kelly E. Activation of cyclic AMP-dependent protein kinase inhibits the desensitization and internalization of metabotropic glutamate receptor 1a and 1b. *Mol. Pharmacol*. 2004; 65 : 1507-1516.
14	Terstappen GC, Pellacani A, Aldegheri L, Graziani F, Carignani C, Pula G, Virginio C. The antidepressant fluoxetine blocks the human small conductance calcium-activated potassium channels SK1, SK2 and SK3. *Neurosci. Lett. 2003; 346* : 85-88.
15	Mundell SJ, Pula G, Carswell K, Roberts PJ, Kelly E. Agonist-induced internalization of metabotropic glutamate receptor 1A: structural determinants for protein kinase C- and G protein-coupled receptor kinase-mediated internalization. *J. Neurochem*. 2003; 84 : 294-304.
16	Mundell SJ, Matharu AL, Pula G, Holman D, Roberts PJ, Kelly E. Metabotropic glutamate receptor 1 internalization induced by muscarinic acetylcholine receptor activation: differential dependency of internalization of splice variants on nonvisual arrestins. *Mol. Pharmacol*. 2002; 61 : 1114-1123.
17	Mundell SJ, Matharu AL, Pula G, Roberts PJ, Kelly E. Agonist-induced internalization of the metabotropic glutamate receptor 1a is arrestin- and dynamin-dependent. *J. Neurochem*. 2001; 78 : 546-551.
18	Terstappen GC, Pula G, Carignani C, Chen MX, Roncarati R. Pharmacological characterisation of the human small conductance calcium-activated potassium channel hSK3 reveals sensitivity to tricyclic antidepressants and antipsychotic phenothiazines. *Neuropharmacology*. 2001; 40 : 772-783.