METHODS
Triple Quad
We use the TSQ Vantage triple stage quadrupole mass spectrometer (Thermo Scientific) for the quantitative analysis of small molecules and peptides. The TSQ Vantage’s new S-Lens ion optics system uses a novel electrostatic field technology to capture virtually every ion and efficiently transfer it into the HyperQuad™ quadrupole mass analyzer. The S-Lens design is a significant advance over high-pressure, skimmer-based ion source designs, as it eliminates mass discrimination and lowers the gas load on the expensive turbo-molecular pumps. This innovation delivers high sensitivity with low noise. The TSQ Vantage is interfaced to a splitless nano LC system (Eksigent) and a TriVersa NanoMate, a chip-based technology from Advion that combines the strengths of liquid chromatography, mass spectrometry, fraction collection, and chip-based infusion in one integrated system.
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